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DRX390009: Illumina HiSeq 4000 paired end sequencing of SAMD00534881
1 ILLUMINA (Illumina HiSeq 4000) run: 218,420 spots, 38M bases, 17.3Mb downloads

Submitted by: OKAYAMA
Study: Genotyping-by-sequencing of melon genetic resources
show Abstracthide Abstract
This project aimed to select the World Melon Core Collection based on genetic variation and population structure characterized by genotyping-by-sequencing for melon genetic resources deposited in the National Agriculture and Food Research Organization (NARO) Genebank, Japan.
Sample: 368
SAMD00534881 • DRS272493 • All experiments • All runs
Organism: Cucumis melo
Library:
Instrument: Illumina HiSeq 4000
Strategy: OTHER
Source: GENOMIC
Selection: Reduced Representation
Layout: PAIRED
Construction protocol: Modified Genotyping-by-sequencing (Elshire et al. 2011) strategy, using Hsp92II or NlaIII restriction enzyme to digest genomic DNA, followed by ligation of custom sequencing adapters including sample-specific barcodes, PCR amplification, and pooling of libraries from 192 samples into the same sequencing run.
Spot descriptor:
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Pipeline: show...hide...
StepPrev Step
1NIL
Runs: 1 run, 218,420 spots, 38M bases, 17.3Mb
Run# of Spots# of BasesSizePublished
DRR404399218,42038M17.3Mb2023-01-01

ID:
26011203

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